The presented work was focused on the study of interactions of azo dyes (model diffusion probes) with cationic biopolymer chitosan. The interactions were realized via diffusion processes in hydrogel media based on thermoreversible agarose. The main aim was study of influence of solution pH on the diffusion process. Interactions of used dyes are based on electrostatic character. The amino group of chitosan interacts with the functional group of chosen anionic dyes (sulfonic group) and thus affects the process of diffusion. Organic anionic dyes Chicago Sky Blue 6B (C.I. 24 410), Sirius Red (C.I. 35 780) and Reactive Blue 49 (C.I. 621 526) were chosen for this experiment. Used diffusion technique was based on monitoring of the time progression of diffusion profile by UV-VIS spectrophotometry. The presented work follows the previous experiments and shows comprehensive view of the reactivity of chitosan and its behavior in different systems. Unsteady diffusion in cuvettes appears to be universal method for the study of reactivity of biopolymers and for the study of transport processes in hydrogel media.Keywords: Diffusion, chitosan, hydrogel, agarose, organic dye, reactivity of biopolymers
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