PREPARATION OF ANTIBACTERIAL ELECTROSPUN SCAFFOLD FOR SKIN CELLS CULTURE

1 GOVORCIN BAJSIC Emi
Co-authors:
1 VECERIC Matea 2 ZDRAVEVA Emilija 2 MIJOVIC Budimir 3 HOLJEVAC GRGURIC Tamara 4 UJCIC Massimo 5 TOMINAC TRCIN Mirna 6 SLIVAC Igor
Institutions:
1 University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, Croatia, EU, egovor@fkit.hr
2 University of Zagreb, Faculty of Textile Technology, Zagreb, Croatia, EU, budimir.mijovic@ttf.hr
3 University of Zagreb,Faculty of MetallurgY, Sisak, Croatia, EU, tholjev@simet.hr
4 Institute of Chemical Processes Fundamentals of the Czech Academy of Science, Prague 6, Czech Republic, EU, ujcic.massimo@gmail.com
5 University Hospital Centre Sestre Milosrdnice, Tissue bank at University Department of Traumatology, University of Zagreb, Zagreb, Croatia, EU, mirna.tomtrcin@gmail.com
6 University of Zagreb, Faculty of Food Technology and Biotechnology, Zagreb, Croatia, EU, islivac@pbf.hr
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
459-464
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
6 views / 2 downloads
Abstract

In this work the function and application of titanium dioxide as a filler in a composite system polycaprolactone/titanium dioxide (PCL/TiO2) was examined. Titanium dioxide was applied by ultrasonic bath on already electrospun PCL fibrous scaffold treated and non-treated with NaOH. A procedure of surface modification of the electrospun PCL fibrous scaffold was made to enhance the interaction of the surface with the TiO2 particles. The surface modification was performed using NaOH for the formation of carboxyl groups on the fibers‘ surfaces. The water contact angle was measured by goniometer to prove the change from hydrophobic to hydrophilic polymer surface. SEM was used to study the morphology structure of the electrospun PCL fibrous scaffold before and after NaOH treatment and introduction of TiO2. The content of TiO2 on the electrospun PCL fibrous scaffold was determined by TGA. After NaOH treatment the surface of the electrospun PCL fibrous scaffolds changed from hydrophobic to hydrophilic. SEM micrographs show that with the sonification of 30 min homogeneous TiO2 particles distribution was obtained, while after sonification of 60 min, the TiO2 particles tend to agglomerate. The modification of the scaffold surface with NaOH enhances the adhesion of the TiO2 filler. TG analysis show that longer treatment of the electrospun PCL fibrous scaffolds in the ultrasonic bath gives lower thermal stability. The time of 30 minutes in the ultrasonic bath is optimal to provide sufficient amount of the TiO2 particles on the electrospun PCL fibrous scaffold.

Keywords: Electrospinning, NaOH, surface modification, titanium dioxide
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