SURFACE MODIFICATION OF ZERO-VALENT IRON NANOPARTICLES BY BIOLOGICAL ACTIVE SUBSTANCE - COMPLEX TESTS

1 Pešková Kristýna
Co-authors:
1 Marková Kristýna 1 Vološčuková Ondřejka 1 Nosek Jaroslav
Institution:
1 Technical University of Liberec, Institute for Nanomaterials, Advanced Technologies and Innovation (CXI), Studentská 2, Liberec, 461 17, Czech Republic; kristyna.peskova@tul.cz, jaroslav.nosek1@tul.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
260-265
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
31 views / 13 downloads
Abstract

Chlorinated solvents such as trichloroethene (TCE) are widespread groundwater contaminants. These contaminants can be transformed by combination of abiotic and biotic methods under anaerobic conditions. Nowadays nanoscale zero-valent iron (nZVI) is used for the treatment of chlorinated compounds via its strong reducing property. Biological reductive dechlorination of chlorinated ethenes (CEs) is contributed by dehalorespiration. The influence of nZVI in combination with carboxymethyl cellulose (CMC) and molasses on the specific dehalorespiring microflora and besides the influence of biological surface modification on reactive properties of iron was tested within this study.Groundwater contaminated with CEs was collected from the chemical factory Spolchemie a.s. Batch tests (reactors) with iron and various concentrations of CMC and molasses were performed. The samples for gas chromatography (GC) analyses were taken regularly after defined time period for determination of CEs concentration and physical-chemical parameters were monitored simultaneously. DNA was extracted after filtration of the tested water and used as a template for a real-time PCR amplification. 16S rDNA gene was used as a total bacterial community marker. Specific genes were used for detection of ongoing reductive dehalogenation (vcrA, bvcA, Dre, DHC-RT and Dsb) and to monitor denitrifying and sulphate reducing bacteria (nirK and apsA).

Keywords: Iron particles, chlorinated solvents, biological surface modification, biological reductive dechlorination, dehalorespiration, molecular genetic analysis
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