INFLUENCE OF NANOPARTICLES ON MICROALGAE DETECTED BY IN VIVO IMAGING

1 PAVELICOVA Kristyna
Co-authors:
1 STREJCKOVA Aneta 1 RANKIC Ivan 1 VANECKOVA Tereza 1 ZELNICKOVA Jaroslava 1 HUSKA Dalibor 1 VACULOVICOVA Marketa
Institution:
1 Department of Chemistry and Biochemistry, Mendel University in Brno, Zemědělská 1, CZ-613 00 Brno, Czech Republic, EU, xpavelic@node.mendelu.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
277-281
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
6 views
Abstract

Fluorescence imaging is an efficient and powerful technique, which has been often utilized for tracking compounds in animals, however its application on plants or other organisms is not very common. In this work, the fluorescence imaging was used to investigate the effect of selected nanoparticles - NPs (ZnO, CdTe, etc.) on microalgae (Scenedesmus quadricauda and Chlorella vulgaris) growth. The growth and metabolic activity of microalgae was influenced by application of nanoparticles into the growing medium (liquid and solid) and therefore the intrinsic fluorescence was affected. Microalgae pigments (chlorophylls, carotenoids) are responsible for the autofluorescence of the species and therefore, the fluorescence can be used as a tool for quick and easy evaluation of expression these compounds. These pigments have main role in light harvesting and minimize photo-oxidative damage of the cells. Such protection mechanism can be disturbed by another stress related compounds like NPs that presumably damage this protection mechanism i) indirectly (reactive oxygen species) and ii) directly (disruption of biomembranes). Owing to broad fluorescence imaging and correlation with total amount of chlorophylls and carotenoids it was possible to evaluate the influence of NPs on individual microalgae just only by in vivo imaging.

Keywords: nanoparticles, Scenedesmus quadricauda, Chlorella vulgaris, fluorescence, abiotic stress, metabolic,
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