ISOLATION OF ARGININE USING MAGHEMITE PARTICLES

1,2 CERNEI Natalia
Co-authors:
1,2 KOPEL Pavel 3 HORKY Pavel 1,2 ZITKA Ondrej 1,2 ADAM Vojtech
Institutions:
1 Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, EU, vojtech.adam@mendelu.cz
2 Central European Institute of Technology, Brno University of Technology, Purkynova 123, CZ-612 00 Brno, Czech Republic, EU
3 Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University in Brno, Zemedelska 1, CZ-613 00 Brno, Czech Republic, EU
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
210-213
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
10 views / 2 downloads
Abstract

In this paper, we report the optimal conditions for the isolation of amino acid arginine from different types of matrix, such as blood, plasma or tissue and their characterization using ion-exchange liquid chromatography with VIS detector. Paramagnetic microparticles able to isolate and immobilize amino acid arginine were prepared and used to preconcentrate arginine for subsequent analysis on ion-exchange liquid chromatography with VIS detector. Commercially available Dowex was covered by maghemite nanoparticles and the composite showed excellent binding and superparamagnetic properties. It was found that the paramagnetic microparticles were able to bind specifically amino acid arginine. Therefore, these paramagnetic microparticles have potential for better isolation of arginine and in future can use for application as a platform of delivery system.

Keywords: Ion Exchange Chromatography, Maghemite microparticles, Amino Acids, arginine
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