DNA STRAND BREAKS AND OXIDATIVE DAMAGE IN LYMPHOCYTES OF WORKERS HANDLING NANOCOMPOSITE MATERIALS.

1 Novotná Božena
Co-authors:
2 Pelclová Daniela 1 Rössner Pavel Jr. 1 Brzicová Táňa 1 Rössnerová Andrea 2 Lischková Lucie 3 Dvořáčková Štěpánka
Institutions:
1 Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic, EU, bozena.novotna@iem.cas.cz, pavel.rossner@iem.cas.cz, tana.brzicova@iem.cas.cz, andrea.rossnerova@iem.cas.cz
2 Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic, EU, daniela@pelclova.cz, Lucie.Lischkova@vfn.cz
3 Technical University in Liberec, Liberec, Czech Republic, EU, stepanka.dvorackova@tul.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
543-547
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
36 views / 3 downloads
Abstract

Many experimental studies have demonstrated the ability of nanoparticles to induce DNA damage and oxidative stress in a number of organs, probably due to the formation of reactive oxygen species. Our knowledge, however, is still limited, especially as regards the persistence of genotoxic effects after long-term exposure to nanoparticles and their health risks. We decided, therefore, to analyze DNA damage in lymphocytes of workers long-term exposed to nanoparticles due to processing of nanocomposite materials.Exposed group involved 20 employees examined pre-shift and post-shift, samples from 20 controls were taken in the morning. Lymphocytes were isolated from heparinized blood on a ficoll gradient and frozen until further processing. An alkaline comet assay with enzymes of excision DNA repair (Fpg and Endo III) was used to detect DNA damage.Within the exposed group, the values of total DNA damage in post-shift samples slightly exceed those in pre-shift ones, but the difference was not statistically significant (% tail DNA 4.47 ± 1.43 vs 3.95 ± 1.23, respectively). Compared to controls, however, the workers showed significantly higher levels of genotoxic damage, both in terms of DNA breakage (% tail DNA 3.08 ± 1.04 vs 1.63 ± 0.50, respectively; p<0.0001), and oxidized bases (% tail DNA 0.88 ± 0.41 vs. 0.57 ± 0.25, respectively; p <0.01). Our preliminary results thus indicate that long term occupational exposure to nanoparticles is associated with an increased risk of DNA damage.

Keywords: Genotoxicity, human, comet assay, nanoparticles, occupational exposure
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