BIOMARKERS OF OXIDATIVE STRESS AND INFLAMMATION IN RESEARCHERS EXPOSED TO NANOPARTICLES BY INHALATION DURING THE HANDLING OF NANOCOMPOSITES

1 PELCLOVÁ Daniela
Co-authors:
2 ŽDÍMAL Vladimír 2 SCHWARZ Jaroslav 3 Komarc Martin 1 VLČKOVÁ Štěpánka 1 FENCLOVÁ Zdenka 1 LISCHKOVÁ Lucie 4 DVOŘÁČKOVÁ Štěpánka 5 RÖSSNEROVÁ Andrea 5 RÖSSNER Pavel Jr.
Institutions:
1 Charles University in Prague and General University Hospital in Prague, First Faculty of Medicine, Department of Occupational Medicine, Prague, Czech Republic, EU, daniela.pelclova@lf1.cuni.cz, stepanka.vlckova@vfn.cz, zdenka.fenclova@lf1.cuni.cz, lucie.lischkova@vfn.cz
2 Institute of Chemical Process Fundamentals of the CAS, Prague, Czech Republic, EU, zdimal@icpf.cas.cz, schwarz@icpf.cas.cz
3 Charles University in Prague and General University Hospital in Prague, First Faculty of Medicine, Institute of Biophysics and Informatics, Prague, Czech Republic, EU, komarc@ftvs.cuni.cz
4 Technical University in Liberec, Faculty of Mechanical Engineering, Department of Machining and Assembly, Liberec, Czech Republic, EU, stepanka.dvorackova@tul.cz
5 Institute of Experimental Medicine, Czech Academy of Sciences, Prague, Czech Republic, EU, andrea.rossnerova@iem.cas.cz, rossner.pavel@iem.cas.cz, fatima.elzeinova@iem.cas.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
531-536
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
4 views / 2 downloads
Abstract

At present, little is known about the health effects in the workers processing nanocomposites. In our study, 20 researchers (41.8±11.4 y/o), handling nanocomposites for 17.8±10.0 years were examined pre-shift and post-shift, together with 21 controls (42.7±11.5 y/o). Biomarkers of oxidative stress derived from lipids, nucleic acids, proteins and markers of inflammation were analyzed in the exhaled breath condensate (EBC). Aerosol exposure was monitored during three nanoparticle generation operations: smelting, welding and nanocomposite machining. Mass concentrations during these operations ranged from 0.120 to 1.840 mg/m3, and median particle number concentrations from 4.8x104 to 5.4x105 particles/cm3. Nanoparticles accounted for 40 to 95% of particles, with Fe and Mn prevailing. Significant elevations were already seen in most oxidative stress markers and in several inflammation markers in the pre-shift samples relative to the controls. Significant associations were found between working in nanocomposite synthesis and the majority of EBC biomarkers. Chronic bronchitis was more frequent in researchers. A minor, but significant post-shift decrease of lung function parameters was found. We conclude that workers in nanocomposite synthesis may be at risk of developing airway disorders with time. From all the markers analyzed in EBC, the following markers were most robust and could be recommended for preventive examinations: 8-hydroxy-2-deoxyguanosine (8-OHdG) and 5-hydroxymethyl uracil (5-OHMeU) from nucleic acids; o-tyrosine (o-Tyr) and 3-nitrotyrosine (3-NOTyr) from proteins; and malondialdehyde and aldehydes C6-C13 from lipids. Among the markers of inflammation, tumor necrosis factor (TNF) and leukotriene B4 appeared to be the most useful.

Keywords: Nanocomposites, exhaled breath condensate, oxidative stress, inflammation, spirometry
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