TI6Al4V is a standard medical titanium alloy. In this study, we compared ground Ti6Al4V discs with nanostructured Ti6Al4V discs prepared using the anodic oxidation method at 30V. Subsequently, both the nanostructured and ground discs were exposed to simulated body fluid (SBF) for 7 days in order to form a hydroxyapatite-like layer. Human mesenchymal stem cells (hMSC) were seeded on the discs in osteogenic differentiation medium and cultivated for 21 days. hMSCs were tested for metabolic activity (MTS assay) and rate of proliferation. The synthesis of type I collagen was evaluated using immunohistochemical staining and confocal microscopy analysis. All samples supported cell adhesion and proliferation. The highest amount of type I collagen was found on nanostructured (N) and ground Ti6Al4V exposed to SBF (TiH) surfaces. Thus, SBF and anodic oxidation positively influenced the growth and osteogenic differentiation of hMSCs.Keywords: Anodic oxidation, hydroxyapatite, mesenchymal stem cell, titanium alloy
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