THE EFFECT OF SIMULATED BODY FLUID ON THE GROWTH AND DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS CULTURED ON NANOSTRUCTURED TI6AL4V SURFACES.

1 FILOVÁ Eva
Co-authors:
1,2 VOLTROVÁ Barbora 3 JAROLÍMOVÁ Petra 4 MATĚJKA Roman 1 LUKÁŠOVÁ Věra 3 FOJT Jaroslav 3 JOSKA Luděk 5 DANIEL Matej
Institutions:
1 Institute of Experimental Medicine of the Czech Academy of Sciences, Prague, Czech Republic, EU, eva.filova@iem.cas.cz
2 Charles University in Prague, Faculty of Science, Czech Republic, EU, barbora.voltrova@iem.cas.cz
3 University of Chemistry and Technology Prague, Faculty of Chemical Technology, Prague, Czech Republic, EU, petra.jarolimova@vscht.cz, jaroslav.fojt@vscht.cz, ludek.joska@vscht.cz
4 Institute of Physiology of the Czech Academy of Sciences, Prague, Czech Republic, EU, roman.matejka@fgu.cas.cz
5 Czech Technical University in Prague, Faculty of Mechanical Engineering, Czech Republic, EU, matej.daniel@fs.cvut.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
300-305
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
19 views / 3 downloads
Abstract

TI6Al4V is a standard medical titanium alloy. In this study, we compared ground Ti6Al4V discs with nanostructured Ti6Al4V discs prepared using the anodic oxidation method at 30V. Subsequently, both the nanostructured and ground discs were exposed to simulated body fluid (SBF) for 7 days in order to form a hydroxyapatite-like layer. Human mesenchymal stem cells (hMSC) were seeded on the discs in osteogenic differentiation medium and cultivated for 21 days. hMSCs were tested for metabolic activity (MTS assay) and rate of proliferation. The synthesis of type I collagen was evaluated using immunohistochemical staining and confocal microscopy analysis. All samples supported cell adhesion and proliferation. The highest amount of type I collagen was found on nanostructured (N) and ground Ti6Al4V exposed to SBF (TiH) surfaces. Thus, SBF and anodic oxidation positively influenced the growth and osteogenic differentiation of hMSCs.

Keywords: Anodic oxidation, hydroxyapatite, mesenchymal stem cell, titanium alloy
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