ON THE FEASIBILITY OF APPLICATION OF BIO-CONJUGATED MOS2 BASED NANOPLATFORM FOR TARGETED CANCER TREATMENT

1 KALOSI Anna
Co-authors:
1,2 ANNUSOVA Adriana 3 LABUDOVA Martina 4 BUGAROVA Nikola 1 SIFFALOVIC Peter 2 ELIASOVA SOHOVA Marianna 1 BENKOVICOVA Monika 1 BODIK Michal 1 JERGEL Matej 1,2 MAJKOVA Eva 4 OMASTOVA Maria 3 PASTOREKOVA Silvia
Institutions:
1 Department of Multilayers and Nanostructures, Institute of Physics, Slovak Academy of Sciences, Bratislava, Slovakia
2 Centre for Advanced Material Application, Slovak Academy of Sciences, Bratislava, Slovakia
3 Department of Cancer Biology, Institute of Virology, Biomedical Research Center, Slovak Academy of Sciences, Bratislava, Slovakia
4 Department of Composite Materials, Polymer Institute, Slovak Academy of Sciences, Bratislava, Slovakia
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
318-323
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
32 views / 10 downloads
Abstract

Surface functionalization of 2D MoS2 materials offers a convenient way to tune their properties and facilitate their application in the field of bio-sensing and nanomedicine. In this study, three different liquid phase exfoliation (LPE) methods were utilized for MoS2 exfoliation: LPE in 45% ethanol solution, LPE in ultrapure water and amino acid intercalation assisted LPE. The three products were bio-conjugated with PEG molecules and through a biotin-avidin-biotin bridge they were functionalized with specific antibody M75 having exclusive affinity towards cancer cells expressing CAIX protein on their membrane. We are aiming to achieve high selectivity towards cancerous cells utilizing an antigen-antibody based cancer detection scheme. This study uses CAIX antigen tagged MDCK cells simulating the cancerous environment and pure MDCK cells as control. Cellular intake studies were performed by confocal Raman microscopy imaging and confocal laser scanning microscopy before and after binding the specific antibody. The preliminary results indicate higher density of internalized nanoplatforms in the case of MDCK cells expressing CAIX compared to the control cells. However, further studies are required to validate these results.

Keywords: MoS2 based nanoplatform, bio-conjugation, antigen-antibody based detection
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