OECT MULTIELECTRODE ARRAY FOR REAL-TIME CELL CULTURE MONITORING

1 SALYK Ota
Co-authors:
2 VÍTEČEK Jan 2 ŠAFAŘÍKOVÁ Eva 1 OMASTA Lukáš 1 STŘÍTESÝ Stanislav 1 Jiří EHLICH 1 VALA Martin 1 WEITER Martin
Institutions:
1 Materials Research Centre, Faculty of Chemistry, Brno University of Technology, Purkyňova 118, 61200 Brno, Czech Republic salyk@fch.vut.cz; xcomastal@fch.vut.cz; xcstritesky@fch.vutbr.cz; xcehlich@fch.vut.cz; vala@fch.vutbr.cz; weiter@fch.vutbr.cz
2 Institute of Biophysics of the Czech Academy of Sciences, Královopolská 135, 61265 Brno, Czech Republic; jan.vitecek@ibp.cz; safarikova.eva@ibp.cz
Conference:
10th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 17th - 19th 2018
Proceedings:
Proceedings 10th International Conference on Nanomaterials - Research & Application
Pages:
336-341
ISBN:
978-80-87294-89-5
ISSN:
2694-930X
Published:
28th February 2019
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
19 views / 11 downloads
Abstract

The significant cost of newly developed pharmacological products is due to the toxicity examination performed on animals in pre-clinical trials. Our ultimate goal is to develop a biosensor array to determine acute cardiotoxicity. It capitalizes on the fact that heart muscle cells (cardiomyocytes) show beating in vitro. Based on the beating characteristics their pathophysiological status can be deducted. In addition to microscopic imaging, the method of monitoring the action and field potential of synchronously beating cardiomyocyte syncytia on multielectrode arrays (MEA) appears to be a suitable method for the investigation of the condition of cardiac cells. For this purpose, MEA on the basis of organic electrochemical transistors (OECTs) has been developed. The organic polymer PEDOT:PSS was utilized to meet the strict surface biocompatibility demand for a surface in contact with media and living cardiac cells. The MEA consisted of a microplate of a 12x8 chimney – well array with transistors on the bottom for cell cultivation. For the fabrication (a screen printing method) primarily a contact field and an organic polymer was used. The device was tested in a simulation mode for the response of the drain current to the voltage pulses applied to the gate electrode up to 100 µV. The response to such gate voltage renders the device sensitive enough to detect the electrical signal of beating cardiomyocytes. Further development is directed towards higher time resolution by means of miniaturization of the transistors’ channel.

Keywords: OECT; screen printing; organic electrochemical transistor; PEDOT:PSS; microplate; multi-electrode array; cell culture
Scroll to Top