EVALUATION OF TWO DIFFERENT METHODS FOR ENDOTOXIN DETECTION IN NANOPARTICLE SUSPENSIONS

1 STEINOVÁ Jana
Co-authors:
1 BOBČÍKOVÁ Kateřina 2 HRISTOV R. Delyan 1 ŠEVCŮ Alena
Institutions:
1 Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Studentská 1402/2, 461 17 Liberec, Czech Republic, EU, jana.steinova@tul.cz
2 Centre for BioNano Interactions, School of Chemistry and Chemical Biology, University College Dublin, Belfield, Dublin 4, Ireland, EU
Conference:
8th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 19th - 21st 2016
Proceedings:
Proceedings 8th International Conference on Nanomaterials - Research & Application
Pages:
627-631
ISBN:
978-80-87294-71-0
ISSN:
2694-930X
Published:
17th March 2017
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
30 views / 14 downloads
Abstract

Nowadays nanomaterials are widely used in industry, medicine, cosmetics, remediation of persistent pollutants, and therefore it is important to test their possible harmful effects on both human health and environment. However, nanoparticles can be often contaminated by bacterial endotoxins (lipopolysaccharides, pyrogens) that can cause false positive results of the (eco)toxicological tests. For this reason, nanoparticle samples should always be screened for endotoxin presence before performing any toxicological studies. Some types of nanoparticles interfere with traditional methods determining the level of endotoxin contamination. The aim of this study was to evaluate two different methods for endotoxin detection and to compare their performance. The first commonly used method, chromogenic LAL assay, is fast, and relatively cheap, but it is known that the test is susceptible to inconclusive results due to nanomaterial interactions. The second one, EndoLISA, is a new, ELISA-based assay in which nanoparticle interference is minimized due to specific binding of endotoxin onto a surface. Our results demonstrate that EndoLISA assay can be less sensitive than chromogenic LAL assay in low endotoxin concentrations, but can be used at higher endotoxin levels in which LAL test cannot be applied.

Keywords: Chromogenic LAL assay, EndoLISA, endotoxin; LPS, nanoparticles
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