INCORPORATION NITRIC OXIDE DONORS INTO FIBROUS MATERIAL INTENDED FOR VASCULAR GRAFTS

1 SAMAN Ales
Co-authors:
1 KLAPSTOVA Andrea 1 HORAKOVA Jana 1 MIKES Petr
Institution:
1 Technical University of Liberec, Faculty Of Textile Engineering, Department of Nonwovens and Nanofibrous Materials, Liberec, Czech Republic, EU
Conference:
8th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 19th - 21st 2016
Proceedings:
Proceedings 8th International Conference on Nanomaterials - Research & Application
Pages:
424-428
ISBN:
978-80-87294-71-0
ISSN:
2694-930X
Published:
17th March 2017
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
18 views / 5 downloads
Abstract

Nitric Oxide (NO) is a very promising molecule suitable for applications in cardiovascular system. NO has many beneficial effects such as anti-thrombogenic and anti-inflammatory properties together with endothelial cell support. Presented work was focused on functionalization of electrospun biodegradable materials by nitric oxide donors for further usage as vascular grafts. Firstly, the optimization of biodegradable nanofibrous layers was carried out. Fibrous sheets were produced from the aliphatic polyester polycaprolactone (PCL) that was blended with nitric oxide donor S-nitrosoacetyl N-penicillamine (SNAPs) in various concentrations of 0.2 wt % and 0.4 wt%. Subsequently, prepared layers with and without NO donors were tested in vitro using endothelial cells and its effect was compared. Nitric oxide release from functionalized layers was detected by colorimetric Griess assay after 1, 2 and 3 days of incubation. Moreover, sterilization methods and their impact for NO release was monitored.

Keywords: Nitric oxide (NO), nanofibers, vascular grafts, Griess assay, scaffolds
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