from the conferences organized by TANGER Ltd. provisional website
Nitric Oxide (NO) is a very promising molecule suitable for applications in cardiovascular system. NO has many beneficial effects such as anti-thrombogenic and anti-inflammatory properties together with endothelial cell support. Presented work was focused on functionalization of electrospun biodegradable materials by nitric oxide donors for further usage as vascular grafts. Firstly, the optimization of biodegradable nanofibrous layers was carried out. Fibrous sheets were produced from the aliphatic polyester polycaprolactone (PCL) that was blended with nitric oxide donor S-nitrosoacetyl N-penicillamine (SNAPs) in various concentrations of 0.2 wt % and 0.4 wt%. Subsequently, prepared layers with and without NO donors were tested in vitro using endothelial cells and its effect was compared. Nitric oxide release from functionalized layers was detected by colorimetric Griess assay after 1, 2 and 3 days of incubation. Moreover, sterilization methods and their impact for NO release was monitored.Keywords: Nitric oxide (NO), nanofibers, vascular grafts, Griess assay, scaffolds
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