MONITORING OF QUANTUM DOT DISTRIBUTION IN PLANTS BY IN VIVO FLUORESCENCE IMAGING

1,2 VANECKOVA Tereza
Co-authors:
1,2 MILOSAVLJEVIC Vedran 1,2 KRYSTOFOVA Olga 1,2 ADAM Vojtech 1,2 VACULOVICOVA Marketa
Institutions:
1 Mendel University in Brno, Department of Chemistry and Biochemistry, Brno, Czech Republic, EU
2 Brno University of Technology, Central European Institute of Technology, Brno, Czech Republic, EU
Conference:
8th International Conference on Nanomaterials - Research & Application, Hotel Voronez I, Brno, Czech Republic, EU, October 19th - 21st 2016
Proceedings:
Proceedings 8th International Conference on Nanomaterials - Research & Application
Pages:
462-466
ISBN:
978-80-87294-71-0
ISSN:
2694-930X
Published:
17th March 2017
Proceedings of the conference were published in Web of Science and Scopus.
Metrics:
35 views / 7 downloads
Abstract

Nanoparticle visualization has extensive potential for their utilization in targeted drug delivery tracking through wide range of biological environments. In this regard, quantum dot nanoparticles may represent a suitable option as they are characterized by very good fluorescent properties. This study is focused on application of quantum dot nanoparticles for fluorescence imaging of plants. A series of in vivo experiments was carried out on leaves of sunflower plant (Helianthus annuus). Leaves were immersed in water solution of CdTe and CdTe/ZnS quantum dots and were monitored (ʎem 535 nm and 600 nm respectively) for 8 hours at time intervals of 60 minutes using In Vivo Xtreme Imaging System (Bruker, Massachusets, USA). Captured images were further processed and the intensity of fluorescence analyzed by Bruker Molecular Imaging Software. Fluorescence imaging of plants encounters difficulties due to autofluorescence of biomolecules present in plants, including chlorophyll, carotene and xanthophyll. However, this can be suppressed by using adequate excitation and emission filters for fluorescence images acquisition. Moreover, visualization of nanoparticles in plants can be enhanced by multispectral imaging and spectral modeling for autofluorescence unmixing. In this study, evaluation regarding optimal characteristics and modifications of quantum dot nanoparticles for fluorescence imaging in plants is provided as well as recommendations on image acquisition setting and further image postprocessing.

Keywords: Quantum dot nanoparticles, in vivo imaging, fluorescence imaging, Helianthus annuus plant, nanoparticle transport visualization
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