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INFLUENCE OF PC3-DERIVED EXOSOMES ON FIBROBLAST PROLIFERATION AND MIGRATION

1 EZATI Masoumeh
Co-authors:
2 SAADATI Arezoo 2 CAO Hoang-Anh 1 ZUMBERG Inna 1 CMIEL Vratislav 3 RICHTERA Lukas
Institutions:
1 Brno University of Technology, Department of Biomedical Engineering, Faculty of Electrical Engineering and Communication, Brno, Czech Republic, EU, Masoumeh.ezati@vut.cz, zumberg@vutbr.cz, Cmiel@vut.cz
2 Brno University of Technology, Central European Institute of Technology, Brno, Czech Republic, EU, saadati@vutbr.cz, Hoang-Anh.Cao@ceitec.vutbr.cz
3 Mendel University in Brno, Department of Chemistry and Biochemistry, Brno, Czech Republic, EU, lukas.richtera@ceitec.vutbr.cz
Conference:
15th International Conference on Nanomaterials - Research & Application, OREA Congress Hotel Brno, Czech Republic, EU, October 18 - 20, 2023
Proceedings:
Proceedings 15th International Conference on Nanomaterials - Research & Application
Pages:
268-272
ISBN:
978-80-88365-15-0
ISSN:
2694-930X
DOI:
https://doi.org/10.37904/nanocon.2023.4802
Published:
13th December 2023
Metrics:
83 views / 37 downloads
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Abstract

Nano-sized exosomes are essential for intercellular communication, especially in cancer biology. Human produces exosomes that may modulate the tumor microenvironment and cellular behaviour. This study examines how human prostate cancer cells (PC3) derived exosomes affect fibroblast proliferation and migration. Ultracentrifugation isolated exosomes from PC3 cell culture supernatants, which were characterized by confocal and transmission electron microscopy (TEM). Fibroblast cells were exposed to these PC3-derived exosomes. MTT [3–(4,5-dimethylthiazol-2yl)-2,5-diphenyl-2H-tetrazoliumbromide] was used to measure fibroblast proliferation, while another assay measured migration. PC3 exosomes appear to boost fibroblast proliferation and migration. Investigations into the molecular mechanisms behind these observations are ongoing. The study shows that PC3-derived exosomes can regulate fibroblast proliferation and migration. This study illuminates the effects of cancer cell-derived exosomes on cellular interactions and therapeutic potential.

Keywords: Human prostate cancer cell (PC3), exosomes, fibroblast proliferation and migration

© This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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